DeFilippes F M
J Virol. 1982 Jul;43(1):136-49. doi: 10.1128/JVI.43.1.136-149.1982.
The cleavage sites for the restriction enzymes Bg/I, HindIII, KpnI, SalI, SmaI, and XhoI were located, from primary data, on the DNA isolated from the WR strain of vaccinia virus. Bg/I and SmaI divide the DNA into five segments which can be isolated by sucrose gradient centrifugation. These large segments provide a convenient means to group segments produced by other enzymes. The construction of physical maps was initiated by identifying the segments at each end of the DNA and then finding segments which were adjacent to these terminal sections. This was accomplished by isolating large shear fragments which contained the covalently linked termini of the DNA. Most of the data needed to derive the maps were obtained by isolating segments produce by one enzyme and then cleaving these individual segments with a second enzyme.
根据原始数据，确定了牛痘病毒WR株分离出的DNA上限制性内切酶Bg/I、HindIII、KpnI、SalI、SmaI和XhoI的切割位点。Bg/I和SmaI将DNA分成五个片段，这些片段可通过蔗糖梯度离心法分离。这些大片段为将其他酶产生的片段进行分组提供了便利的方法。物理图谱的构建始于识别DNA两端的片段，然后找到与这些末端片段相邻的片段。这是通过分离包含DNA共价连接末端的大剪切片段来实现的。推导图谱所需的大部分数据是通过分离一种酶产生的片段，然后用另一种酶切割这些单个片段获得的。
