Masker W E, Kuemmerle N B
J Virol. 1980 Jan;33(1):330-9. doi: 10.1128/JVI.33.1.330-339.1980.
A system capable of in vitro packaging of exogenous bacteriophage T7 DNA has been used to monitor the biological activity of DNA replicated in vitro. This system has been used to follow the effects of UV radiation on in vitro replication and recombination. During the in vitro replication process, a considerable exchange of genetic information occurs between T7 DNA molecules present in the reaction mixture. This in vitro recombination is reflected in the genotype of the T7 phage produced after in vitro encapsulation; depending on the genetic markers selected, recombinants can comprise nearly 20% of the total phage production. When UV-irradiated DNA is incubated in this system, the amount of in vitro synthesis is reduced and the total amount of viable phage produced after in vitro packaging is diminished. In vitro recombination rates are also lower when the participating DNA molecules have been exposed to UV. However, biochemical and genetic measurements confirmed that there is little or no transfer of pyrimidine dimers from irradiated DNA into undamaged molecules.
一种能够对外源噬菌体T7 DNA进行体外包装的系统已被用于监测体外复制的DNA的生物学活性。该系统已被用于追踪紫外线辐射对体外复制和重组的影响。在体外复制过程中，反应混合物中存在的T7 DNA分子之间会发生大量的遗传信息交换。这种体外重组反映在体外包装后产生的T7噬菌体的基因型中；根据所选择的遗传标记，重组体可占噬菌体总产量的近20%。当紫外线照射的DNA在该系统中孵育时，体外合成量减少，体外包装后产生的活噬菌体总量减少。当参与的DNA分子暴露于紫外线时，体外重组率也较低。然而，生化和遗传学测量证实，嘧啶二聚体从受辐照的DNA转移到未受损分子中的情况很少或没有。
