Rayssiguier C, Kozinski A W, Doermann A H
J Virol. 1980 Aug;35(2):451-65. doi: 10.1128/JVI.35.2.451-465.1980.
A physicochemical study was made of the replication and transmission of UV-irradiated T4 genomes. The data presented in this paper justify the following conclusions. (i) For both low and high multiplicity of infection there was abundant replication from UV-irradiated parental templates. It exceeded by far the efficiency predicted by the hypothesis that a single lethal hit completely prevents replication of the killed phage DNA: i.e., some dead phage particles must replicate parts of thier DNA. (ii) Replication of the UV-irradiated DNA was repetitive as shown by density reversal experiments. (iii) Newly synthesized progeny DNA originating from UV-irradiated templates appeared as significantly shorter segments of the genomes than progeny DNA produced from non-UV-irradiated templates. A good correlation existed between the number of UV hits and the number of random cuts that would be needed to reduce replication fragments to the length observed. (iv) The contribution of UV-irradiated parental DNA among progeny phage in multiplicity reactivation was disposed in shorter subunits than was the DNA from unirradiated parental phage. It is important to emphasize that it was mainly in the form of replicative hybrid. These conclusions appear to justify excluding interparental recombination as a prerequisite for multiplicity reactivation. They lead directly to some form of partial replica hypothesis for multiplicity reactivation.
对紫外线照射的T4基因组的复制和传递进行了物理化学研究。本文给出的数据支持以下结论。(i) 对于低感染复数和高感染复数，紫外线照射的亲本模板都有大量复制。这远远超过了单一致死性损伤完全阻止被杀死噬菌体DNA复制这一假设所预测的效率：也就是说，一些死亡的噬菌体颗粒必须复制其部分DNA。(ii) 如密度反转实验所示，紫外线照射DNA的复制是重复性的。(iii) 源自紫外线照射模板的新合成子代DNA在基因组中表现为明显更短的片段，比未受紫外线照射模板产生的子代DNA短。紫外线损伤的数量与将复制片段减少到观察到的长度所需的随机切割数量之间存在良好的相关性。(iv) 在多重复活中，子代噬菌体中紫外线照射亲本DNA的贡献以比未照射亲本噬菌体的DNA更短的亚基形式存在。需要强调的是，它主要以复制性杂种的形式存在。这些结论似乎证明可以排除亲本间重组作为多重复活的先决条件。它们直接导致了某种形式的多重复活部分复制假说。
