Dodd R Y
Transfusion. 1980 Mar-Apr;20(2):212-6. doi: 10.1046/j.1537-2995.1980.20280169963.x.
Hepatitis B surface antigen (HBsAg) may be insolubilized onto a controlled pore glass (CPG) matrix by simple adsorption from acidified serum or plasma. The resultant complex has the properties of an HBsAg-specific immunoadsorbent. In this study, CPG-HBsAg complexes have been used to adsorb anti-HBs from standard immune serum globulin. Specific antibodies were eluted with acidic or chaotropic buffers. Recoveries ranging from 29 to 46 per cent, with purifications of approximately 1000-fold were obtained. Two procedures designed to inactivate hepatitis B virus had no effect upon the immunoadsorbent properties of the CPG-HBsAg complex. The possibility of using this simple method to prepare hepatitis B immune globulin is discussed.
通过从酸化的血清或血浆中简单吸附，可将乙型肝炎表面抗原（HBsAg）固定在可控孔径玻璃（CPG）基质上。所得复合物具有HBsAg特异性免疫吸附剂的特性。在本研究中，CPG-HBsAg复合物已用于从标准免疫血清球蛋白中吸附抗-HBs。用酸性或离液序列高的缓冲液洗脱特异性抗体。回收率在29%至46%之间，纯化倍数约为1000倍。两种旨在灭活乙型肝炎病毒的程序对CPG-HBsAg复合物的免疫吸附特性没有影响。讨论了使用这种简单方法制备乙型肝炎免疫球蛋白的可能性。
