Kosiakov P N, Rovnova Z I, Isaeva E I, Platonova A L, Pankratov V S
Vopr Virusol. 1976 May-Jun(3):301-8.
The presence of three antigenic determinants was demonstrated by the method of selective adsorption of inhibitor-free sera (HI test) and complement-fixation test (CFT) in hemagglutinins (HA) of influenza A/Port Chalmers/73 and A/USSR/053/74 viruses. These variants with closely similar antigenic composition have two antigenic determinants related with NA of A/England/42/72 virus, whereas the third antigenic determinant is specific only for them and is lacking in A/England/42/72 virus NA. According to the results obtained the antigenic composition of HA of these viruses may be designated in the following way: A/England/42/72--H3 H6 H7, A/Port Chalmers/73 and A/USSR/053/74--H3 H7 H8. A method is suggested for preparation of monospecific serum to the antigenic determinant H3 common to all variants of the Hong Kong variety of influenza virus. This serum is free from nonspecific inhibitors, contains no antibody to the host cell antigens and may be used both in CFT and HI test for rapid identification of new influenza virus isolates.
通过对甲型流感病毒A/查尔默斯港/73株和A/苏联/053/74株血凝素（HA）进行无抑制剂血清的选择性吸附法（血凝抑制试验）和补体结合试验（CFT），证实了三种抗原决定簇的存在。这些抗原组成极为相似的病毒变种具有两个与A/英格兰/42/72病毒的神经氨酸酶（NA）相关的抗原决定簇，而第三个抗原决定簇仅为它们所特有，在A/英格兰/42/72病毒的NA中不存在。根据所得结果，这些病毒HA的抗原组成可按以下方式表示：A/英格兰/42/72——H3 H6 H7，A/查尔默斯港/73和A/苏联/053/74——H3 H7 H8。本文提出了一种制备针对香港型流感病毒所有变种共有的抗原决定簇H3的单特异性血清的方法。该血清不含非特异性抑制剂，不含有针对宿主细胞抗原的抗体，可用于补体结合试验和血凝抑制试验，以快速鉴定新分离的流感病毒。
