Berglindh T, Dibona D R, Ito S, Sachs G
Am J Physiol. 1980 Mar;238(3):G165-76. doi: 10.1152/ajpgi.1980.238.3.G165.
The site of acid secretion in the gastric mucosa has been inferred, but never proven. Using differential interference-contrast (Nomarski) microscopy an expansion of intracellular vacuoles was observed in the parietal cells of living rabbit gastric glands following histamine stimulation. A similar vacuolization occurring only in part of a parietal cell population could be induced by high concentrations of accumulated weak base, aminopyrine, in the absence of secretagogue. In high-K+ medium, 10(-3) M aminopyrine induced massive vacuolization in all parietal cells, consistent with the strong effect of high K+ in stimulating aminopyrine uptake by isolated glands. Electron micrographs showed that the apparent vacuoles correspond to the secretory canaliculi in various stages of swelling. Acridine orange, a fluorescent dye which is distributed across natural membranes as a function of a pH gradient and binds in a multimolecular fashion (stacking) to negative sites, was accumulated by gastric glands as a function of acid secretion. Visualization of such glands by fluorescence or a combination of Nomarski and fluorescence microscopy showed a red fluorescence in the expanding secretory canaliculi that was in sharp contrast to the green fluorescence in the rest of the cell. From these data it is concluded that the site of acid secretion is indeed the secretory canaliculus of the parietal cell. It is also possible that the formation of secretory canaliculi may be induced osmotically and that the peripheral "parietal" position and triangular shape of the parietal cell is necessary to allow expansion and oriented apical flow of HCl.
胃黏膜中酸分泌的部位已被推断出来，但从未得到证实。使用微分干涉相差（诺马斯基）显微镜观察到，组胺刺激后，活兔胃腺壁细胞内的细胞内液泡出现扩张。在没有促分泌剂的情况下，高浓度积累的弱碱氨基比林可诱导仅部分壁细胞群体出现类似的空泡化。在高钾培养基中，10⁻³ M的氨基比林可诱导所有壁细胞出现大量空泡化，这与高钾对分离腺体摄取氨基比林的强烈刺激作用一致。电子显微镜照片显示，明显的液泡对应于处于不同肿胀阶段的分泌小管。吖啶橙是一种荧光染料，它根据pH梯度分布在天然膜上，并以多分子方式（堆积）与负性位点结合，胃腺会根据酸分泌情况积累吖啶橙。通过荧光或诺马斯基显微镜与荧光显微镜相结合的方式观察这些腺体，可发现扩张的分泌小管中呈现红色荧光，这与细胞其他部分的绿色荧光形成鲜明对比。从这些数据可以得出结论，酸分泌的部位确实是壁细胞的分泌小管。分泌小管的形成也可能是由渗透作用诱导的，并且壁细胞的外周“壁”位和三角形形状对于允许盐酸的扩张和定向顶端流动是必要的。
