胃酸分泌的细胞位点。

DiBona D R, Ito S, Berglindh T, Sachs G

Proc Natl Acad Sci U S A. 1979 Dec;76(12):6689-93. doi: 10.1073/pnas.76.12.6689.

Isolated gastric glands of the rabbit were examined both with differential interference-contrast microscopy and with electron microscopy to describe the morphologic correlates of acid secretion. Stimulation of the glands with histamine resulted in the development of intracellular spaces within the parietal cells. A similar transformation was produced by addition of 1 mM aminopyrine, whether the weak base was added in the presence of normal-K+ (5.4 mM) or high-K+ (108 mM) solutions. The intracellular space was compatible with the expanded canaliculus described in stimulated parietal cells. Confirmation that the space produced by histamine is the site of acid secretion was gained by combining fluorescence and interference-contrast methods in the presence of the dye acridine orange, which displays a pH-dependent metachromasia in its emission spectrum. Human gastrin I resulted in an observable discharge of peptic granules.

用微分干涉相差显微镜和电子显微镜检查了兔的分离胃腺，以描述酸分泌的形态学相关因素。用组胺刺激腺体导致壁细胞内出现细胞内间隙。加入1 mM氨基比林也产生了类似的转变，无论这种弱碱是在正常钾离子（5.4 mM）还是高钾离子（108 mM）溶液存在的情况下加入。细胞内间隙与刺激的壁细胞中描述的扩张小管相符。通过在吖啶橙染料存在的情况下结合荧光和干涉相差方法，证实了组胺产生的间隙是酸分泌的部位，吖啶橙在其发射光谱中显示出pH依赖性的异染性。人胃泌素I导致可观察到的胃蛋白酶颗粒释放。