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呼肠孤病毒子代亚病毒颗粒中的mRNA加帽酶被掩盖。

mRNA capping enzymes are masked in reovirus progeny subviral particles.

作者信息

Skup D, Millward S

出版信息

J Virol. 1980 May;34(2):490-6. doi: 10.1128/JVI.34.2.490-496.1980.

DOI:10.1128/JVI.34.2.490-496.1980
PMID:6246277
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC288728/
Abstract

We examined the enzyme activities associated with progeny subviral particles isolated from L-cells infected with reovirus at 12 h postinfection. Activities normally present in reovirus cores were also found to be present in the progeny subviral particles, with the exception of the capping enzymes. The methylase and guanyl transferase activities, which constitute the capping system, were present in a masked form that could be activated by chymotrypsin digestion. The appearance of these progeny subviral particles in infected cells coincided with the time when mRNA synthesis was maximal, suggesting that viral mRNA synthesized at later times is uncapped.

摘要

我们检测了感染呼肠孤病毒12小时后从L细胞中分离出的子代亚病毒颗粒相关的酶活性。除了加帽酶外，通常存在于呼肠孤病毒核心中的活性也存在于子代亚病毒颗粒中。构成加帽系统的甲基化酶和鸟苷酸转移酶活性以一种可被胰凝乳蛋白酶消化激活的隐蔽形式存在。这些子代亚病毒颗粒在感染细胞中的出现与mRNA合成达到最大值的时间一致，这表明在较晚时间合成的病毒mRNA是未加帽的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/929f/288728/fb81325d3437/jvirol00173-0199-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/929f/288728/7a9f1ff213e7/jvirol00173-0198-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/929f/288728/fb81325d3437/jvirol00173-0199-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/929f/288728/7a9f1ff213e7/jvirol00173-0198-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/929f/288728/fb81325d3437/jvirol00173-0199-a.jpg

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1
mRNA capping enzymes are masked in reovirus progeny subviral particles.呼肠孤病毒子代亚病毒颗粒中的mRNA加帽酶被掩盖。
J Virol. 1980 May;34(2):490-6. doi: 10.1128/JVI.34.2.490-496.1980.
2
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本文引用的文献

1
Reovirus progeny subviral particles synthesize uncapped mRNA.呼肠孤病毒子代亚病毒颗粒合成无帽mRNA。
J Virol. 1980 May;34(2):497-505. doi: 10.1128/JVI.34.2.497-505.1980.
2
Reovirus-induced modification of cap-dependent translation in infected L cells.呼肠孤病毒诱导的感染L细胞中帽依赖性翻译的修饰。
Proc Natl Acad Sci U S A. 1980 Jan;77(1):152-6. doi: 10.1073/pnas.77.1.152.
3
Reovirus: RNA polymerase activity in purified virions.呼肠孤病毒：纯化病毒颗粒中的RNA聚合酶活性
Viruses. 2021 Feb 13;13(2):294. doi: 10.3390/v13020294.
4
How Many Mammalian Reovirus Proteins are involved in the Control of the Interferon Response?有多少种哺乳动物呼肠孤病毒蛋白参与干扰素反应的调控？
Pathogens. 2019 Jun 21;8(2):83. doi: 10.3390/pathogens8020083.
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Cell Entry-Independent Role for the Reovirus μ1 Protein in Regulating Necroptosis and the Accumulation of Viral Gene Products.细胞进入非依赖性的呼肠孤病毒 μ1 蛋白在调控坏死性凋亡和病毒基因产物积累中的作用。
J Virol. 2019 May 15;93(11). doi: 10.1128/JVI.00199-19. Print 2019 Jun 1.
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Synthesis and Translation of Viral mRNA in Reovirus-Infected Cells: Progress and Remaining Questions.呼肠孤病毒感染细胞中病毒 mRNA 的合成与翻译：进展与遗留问题。
Viruses. 2018 Nov 27;10(12):671. doi: 10.3390/v10120671.
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