Zarbl H, Skup D, Millward S
J Virol. 1980 May;34(2):497-505. doi: 10.1128/JVI.34.2.497-505.1980.
Reovirus progeny subviral particles were isolated from L-cells at late times postinfection. It has been shown (D. Skup and S. Millward, J. Virol. 34: 490--496, 1980) that these progeny subviral particles have masked capping enzymes, indicating that mRNA synthesized by these particles should be uncapped. When progeny subviral particles were used for mRNA synthesis in vitro, they failed to incorporate the beta-phosphate of [beta-32P]GTP into the 5' terminal. Direct analysis of reovirus mRNA synthesized by progeny subviral particles in the presence of either [alpha-32P]GTP or [alpha-32P]CTP indicated that the 5' terminal was uncapped, having the structure pGpC... The implications of this finding to the reovirus replicative cycle are discussed.
在感染后期从L细胞中分离出呼肠孤病毒子代亚病毒颗粒。研究表明（D. Skup和S. Millward，《病毒学杂志》34: 490 - 496，1980），这些子代亚病毒颗粒具有被掩盖的加帽酶，这表明由这些颗粒合成的mRNA应该是未加帽的。当子代亚病毒颗粒用于体外mRNA合成时，它们未能将[β - 32P]GTP的β - 磷酸基团掺入5'末端。在[α - 32P]GTP或[α - 32P]CTP存在的情况下，对由子代亚病毒颗粒合成的呼肠孤病毒mRNA进行直接分析表明，5'末端未加帽，其结构为pGpC... 本文讨论了这一发现对呼肠孤病毒复制周期的影响。
