通过质粒拯救分离鸡胸苷激酶基因。
Isolation of the chicken thymidine kinase gene by plasmid rescue.
作者信息
Perucho M, Hanahan D, Lipsich L, Wigler M
出版信息
Nature. 1980 May 22;285(5762):207-10. doi: 10.1038/285207a0.
DOI:10.1038/285207a0
PMID:6246445
Abstract
We have used the bacterial plasmid pBR322 as a vehicle to isolate genes coding for selectable markers from higher eukaryotes. In this way, we have obtained the chicken thymidine kinase (tk) gene as a 2.2-kilobase EcoRI/HindIII insert in BR322. The cloned gene transforms tk- animal cells with an efficiency equal to that of the cloned herpes simplex virus-1 tk gene.
摘要
我们已将细菌质粒pBR322用作载体，从高等真核生物中分离编码选择标记的基因。通过这种方式，我们获得了鸡胸苷激酶（tk）基因，它以一个2.2千碱基的EcoRI/HindIII片段插入到BR322中。克隆的该基因转化tk-动物细胞的效率与克隆的单纯疱疹病毒1型tk基因相同。
Zotero 插件