Kitchener G, Hayman M J
Proc Natl Acad Sci U S A. 1980 Mar;77(3):1637-41. doi: 10.1073/pnas.77.3.1637.
The gag-related proteins found in cells transformed by avian erythroblastosis virus (AEV) and the avian myelocytomatosis viruses MC29 and CMII have been compared by tryptic peptide fingerprinting. A comparison of the methionine-containing tryptic peptides of the AEV 75-kilodalton protein, the CMII 90-kilodalton protein, and the MC29 110-kilodalton protein with the gag gene product Pr76 of their naturally occurring helper leukemia viruses enabled us to distinguish those peptides related to the gag gene from the non-gag-related peptides. The 12 non-gag peptides found in the AEV 75-kilodalton protein were unique to this protein and not found in the MC29 110-kilodalton or CMII 90-kilodalton proteins. In contrast, the MC29 110-kilodalton protein shared two methionine-containing non-gag tryptic peptides with the CMII 90-kilodalton protein. When these experiments were repeated with [14C]lysine and [14C]arginine as the labeled amino acids, the MC29 110-kilodalton protein and the CMII 90-kilodalton protein were found to share 30 out of approximately 40 non-gag-related peptides. These results demonstrate that viruses with a similar transformation spectrum synthesize related proteins and suggest that the gag-related proteins represent the transforming proteins of the replication-defective avian leukemia viruses.
通过胰蛋白酶肽指纹图谱法，对在禽成红细胞增多症病毒（AEV）以及禽骨髓细胞瘤病毒MC29和CMII转化的细胞中发现的与gag相关的蛋白质进行了比较。将AEV 75千道尔顿蛋白、CMII 90千道尔顿蛋白和MC29 110千道尔顿蛋白中含甲硫氨酸的胰蛋白酶肽段，与其天然存在的辅助白血病病毒的gag基因产物Pr76进行比较，使我们能够区分与gag基因相关的肽段和与gag无关的肽段。在AEV 75千道尔顿蛋白中发现的12个非gag肽段是该蛋白所特有的，在MC29 110千道尔顿蛋白或CMII 90千道尔顿蛋白中未发现。相反，MC29 110千道尔顿蛋白与CMII 90千道尔顿蛋白共享两个含甲硫氨酸的非gag胰蛋白酶肽段。当以[14C]赖氨酸和[14C]精氨酸作为标记氨基酸重复这些实验时，发现MC29 110千道尔顿蛋白和CMII 90千道尔顿蛋白在大约40个与gag无关的肽段中共享30个。这些结果表明，具有相似转化谱的病毒合成相关蛋白质，并表明与gag相关的蛋白质代表复制缺陷型禽白血病病毒的转化蛋白。
Zotero 插件
