Brown N L, McClelland M, Whitehead P R
Gene. 1980 Apr;9(1-2):49-68. doi: 10.1016/0378-1119(80)90166-3.
A new class II restriction endonuclease, HgiAI has been partially purified from Herpetosiphon giganteus HP1023. The enzyme activity has been characterized and shown to recognize the family of related hexanucleotide sequences (Formula: see text) where the second and fifth nucleotide pairs are A:T pairs in either orientation. Cleavage occurs as shown, to give DNA fragments with 3'-terminal tetranucleotide extensions. The recognition sites of the enzymes SacI and SstI (Formula: see text) form a subset of the recognition site of HgiAI. One of the four possible tetranucleotide 3'-extensions (cohesive ends), generated by HgiAI is identical with those generated by SacI and SstI, another is identical with that of PstI. HgiAI should be useful for molecular cloning.
一种新的II类限制性内切酶HgiAI已从巨大管吸放线菌HP1023中部分纯化出来。该酶的活性已得到表征，显示其识别相关六核苷酸序列家族（公式：见正文），其中第二和第五核苷酸对为任意方向的A:T对。切割如图所示，产生具有3'-末端四核苷酸延伸的DNA片段。SacI和SstI酶的识别位点（公式：见正文）构成了HgiAI识别位点的一个子集。HgiAI产生的四种可能的四核苷酸3'-延伸（粘性末端）之一与SacI和SstI产生的相同，另一个与PstI的相同。HgiAI在分子克隆中应该会很有用。
