Shinomiya T, Kobayashi M, Sato S
Nucleic Acids Symp Ser. 1980(8):s181-4.
A second site specific endonuclease with a novel specificity has been isolated from Thermus thermophilus strain 111 and named Tth111II. The enzyme is active at temperature up to 80 degrees C and requires Mg2+ or Mn2+ for activity. Tth111II cleaves phi X174RFDNA into 11 fragments. From the analysis of 5' terminal sequences of the phi X174RFDNA fragments produced by Tth111II action, it was concluded that Tth111II recognized the DNA sequence (See formula in text) and cleaved the sites as indicated by arrows.
一种具有新型特异性的第二位点特异性内切核酸酶已从嗜热栖热菌111菌株中分离出来，并命名为Tth111II。该酶在高达80摄氏度的温度下具有活性，且活性需要Mg2+或Mn2+。Tth111II将φX174RFDNA切割成11个片段。通过对Tth111II作用产生的φX174RFDNA片段的5'末端序列分析，得出Tth111II识别DNA序列（见文中公式）并按箭头所示位点切割的结论。
