Purvis I J, Moseley B E
Nucleic Acids Res. 1983 Aug 25;11(16):5467-74. doi: 10.1093/nar/11.16.5467.
A type II restriction endonuclease, DraI, isolated from Deinococcus radiophilus ATCC 27603 recognises the palindromic hexanucleotide sequence (formula; see text) and cleaves it, as indicated by the arrows, to produce blunt-ended fragments. The yield of enzyme is 100 to 1000 times that of the only other known type II restriction endonuclease that recognises a sequence composed solely of A:T basepairs, the isoschizomer AhaIII (1). Ultraviolet irradiation of the DNA substrate at relatively low doses inhibits the activity of DraI by "protecting" the recognition sequence and this may be exploited to give control of partial digestion of DNA by DraI.
从嗜放射栖热菌ATCC 27603中分离出的II型限制性内切酶DraI识别回文六核苷酸序列（分子式；见正文），并如箭头所示将其切割，产生平端片段。该酶的产量是另一种已知的仅识别由A:T碱基对组成序列的II型限制性内切酶——同裂酶AhaIII产量的100至1000倍（1）。以相对低剂量对DNA底物进行紫外线照射，通过“保护”识别序列来抑制DraI的活性，这可用于控制DraI对DNA的部分消化。
