Macnaughton M R, Davies H A
J Gen Virol. 1980 Apr;47(2):365-72. doi: 10.1099/0022-1317-47-2-365.
Two distinct types of avian infectious bronchitis virus (IBV) particles were isolated on sucrose density gradients. The higher density particles banded at 1.18 g/ml, had typical coronavirus morphology and contained all the structural polypeptides and a complete genome. The less dense particles of density 1.13 g/ml appeared to have typical coronavirus morphology, although they were much more flattened than the more dense particles. Furthermore, these particles lacked the ribonucleoprotein polypeptide and the genome, although all the other polypeptides were present in the same amounts as in the denser particles.
在蔗糖密度梯度上分离出两种不同类型的禽传染性支气管炎病毒(IBV)颗粒。密度较高的颗粒在1.18 g/ml处形成条带,具有典型的冠状病毒形态,包含所有结构多肽和完整的基因组。密度为1.13 g/ml的密度较低的颗粒似乎也具有典型的冠状病毒形态,尽管它们比密度较高的颗粒更扁平。此外,这些颗粒缺乏核糖核蛋白多肽和基因组,尽管所有其他多肽的含量与密度较高的颗粒相同。