Kravtsova V V, Kravtsov A V, Iaroshenko N A
Ukr Biokhim Zh (1978). 1983 Jul-Aug;55(4):392-7.
Stability of Na+, K+-ATPase (EC 3.6.1.3) of the brain microsomal fraction towards thermoinactivation was studied under the effect of surfactants--alkyl sulphates with the C8-C15 chain length of a hydrocarbon radical, desoxycholate, triton X-100, twin-80 and digitonin. It is shown that NaCl treatment of the enzyme before thermoinactivation, and also in the presence of surfactants, increases its resistance to the effect of temperature (octyl and decyl sulphates are an exception, in their presence transport ATPase preincubated with NaCl is completely inactivated). In the presence of most surfactants used in the maximum activating concentrations the activity of Na+, K+-ATPase remained at a considerably high level (80-110% of the initial one) and did not change essentially under NaCl pretreatment of the enzyme. Besides octyl and decyl sulphate, desoxycholate, was also an exception, in the presence of NaCl it inhibited sharply the Na+, K+-ATPase activity. An interrelation is found between the ability of surfactants to decrease the activity of transport ATPase and to solubilize proteins and lipids from the microsomal fraction.
在表面活性剂（碳氢链长度为C8 - C15的烷基硫酸盐、脱氧胆酸盐、曲拉通X - 100、吐温 - 80和洋地黄皂苷）的作用下，研究了脑微粒体部分的Na⁺,K⁺ - ATP酶（EC 3.6.1.3）对热失活的稳定性。结果表明，在热失活前以及存在表面活性剂的情况下，用NaCl处理该酶可增强其对温度作用的抗性（辛基和癸基硫酸盐除外，在它们存在时，与NaCl预孵育的转运ATP酶会完全失活）。在大多数以最大激活浓度使用的表面活性剂存在下，Na⁺,K⁺ - ATP酶的活性保持在相当高的水平（初始活性的80 - 110%），并且在酶经NaCl预处理后基本不变。除了辛基和癸基硫酸盐外，脱氧胆酸盐也是一个例外，在NaCl存在时它会急剧抑制Na⁺,K⁺ - ATP酶的活性。发现表面活性剂降低转运ATP酶活性以及从微粒体部分溶解蛋白质和脂质的能力之间存在相互关系。
