Gordon J M, Walker C B, Goodson J M, Socransky S S
Antimicrob Agents Chemother. 1980 Feb;17(2):193-8. doi: 10.1128/AAC.17.2.193.
An increased interest in the clinical use of antibiotics as an adjunct to periodontal therapy has created a need to determine antibiotic concentrations in fluid obtained from the gingival crevice. For this purpose, an increase in sensitivity beyond that possible with current tetracycline assays is essential because sample volumes of gingival fluid typically obtained are less than 0.5 microliter. This report describes the development of an agar-diffusion assay technique capable of measuring the concentration of tetracycline in samples of gingival crevice fluid in the range of 0.1 to 4.0 microgram/ml. The assay will detect amounts of tetracycline in gingival crevice fluid samples as low as 50 pg. The high sensitivity of this assay was achieved by optimizing the medium depth, inoculum density, agar concentration, pH, period of prediffusion, and selection of basal medium. Use of this assay indicated that the concentration of tetracylcine in gingival crevice fluid was greater than that found in blood and persisted at elevated levels for longer periods.
人们对抗生素作为牙周治疗辅助手段的临床应用兴趣日增，这就需要测定从龈沟获取的液体中的抗生素浓度。为此，灵敏度必须高于目前四环素检测方法所能达到的水平，因为通常获取的龈沟液样本体积小于0.5微升。本报告描述了一种琼脂扩散检测技术的开发，该技术能够测量龈沟液样本中四环素的浓度，范围为0.1至4.0微克/毫升。该检测方法能检测出低至50皮克的龈沟液样本中的四环素含量。通过优化培养基深度、接种物密度、琼脂浓度、pH值、预扩散时间以及基础培养基的选择，实现了该检测方法的高灵敏度。使用该检测方法表明，龈沟液中四环素的浓度高于血液中的浓度，且在较长时间内保持在较高水平。
