Offenbacher S, Farr D H, Goodson J M
J Clin Periodontol. 1981 Aug;8(4):359-67. doi: 10.1111/j.1600-051x.1981.tb02045.x.
A method for the detection of prostaglandin E (PGE) in crevicular fluid has been developed which provides a sensitive, noninvasive technique for measurement of local concentrations of this mediator of inflammation. Assay sensitivity sufficient for the detection of 4 picograms of PGE2 was achieved by utilizing a high-affinity anti-PGE2 antibody, a solid-state second antibody and low isotope concentrations. The method permits detection of concentrations equivalent to 10(-8) M PGE2 in 1 microliter of crevicular fluid. Crevicular fluid PGE (CFPGE) concentrations were determined in samples from 12 patients with periodontal disease. Patients with periodontitis had significantly higher mean CFPGE concentrations than patients with gingivitis (179.5 +/- 51.4 pg/microliter vs 32.1 +/- 15.5 pg/microliter, mean +/- SEM). Periodontitis sites were selected on the basis of clinical and radiographic evidence of periodontal destruction. Some sites displayed low CFPGE levels, while others had CFPGE concentrations which were elevated tenfold, suggesting the presence of both inactive and active periodontal lesions. CFPGE levels greater than 100 pg/microliter were positively associated with gingival erythema and pain on probing.
已开发出一种检测龈沟液中前列腺素E（PGE）的方法，该方法为测量这种炎症介质的局部浓度提供了一种灵敏、非侵入性的技术。通过使用高亲和力抗PGE2抗体、固态二抗和低同位素浓度，实现了足以检测4皮克PGE2的检测灵敏度。该方法能够检测出1微升龈沟液中相当于10（-8）M PGE2的浓度。测定了12例牙周病患者样本中的龈沟液PGE（CFPGE）浓度。牙周炎患者的平均CFPGE浓度显著高于牙龈炎患者（179.5±51.4皮克/微升对32.1±15.5皮克/微升，平均值±标准误）。根据牙周破坏的临床和影像学证据选择牙周炎部位。一些部位的CFPGE水平较低，而其他部位的CFPGE浓度升高了10倍，这表明存在非活动性和活动性牙周病变。CFPGE水平大于100皮克/微升与牙龈红斑和探诊疼痛呈正相关。
