Wakabayashi K, Minegishi T, Yorozu Y, Igarashi M, Ichinoe K
Endocrinol Jpn. 1980 Feb;27(1):87-93. doi: 10.1507/endocrj1954.27.87.
After a single PMS (50 IU) injection to 25-day-old rats, FSH receptor content of the ovarian tissue increased progressively for 4 days, then showed a tendency to decrease, while LH receptor content remained unchanged for 3 days, then gradually increased. From these facts, we established a radioreceptor assay system, employing 3,000 rpm precipitates of homogenates of the ovaries obtained 3 days after PMS injection as the receptor preparation. The dissociahe standard curve was obtained with 0.125--16 ng/tube of NIAMDD rat FSH I-3. Purified preparations, NIAMDD rat LH I-4 and NIAMDD rat TSH I-4 influenced the binding only at high concentrations possibly owing to FSH contamination. When the anterior pituitary homogenates obtained from rats in the various physiological states were assayed by this system, the intra-assay coefficient of variation and inter-assay coefficient of variation were 7.5% and 13.7%, respectively, and the assay values were well correlated with those obtained by radioimmunoassay (r = 0.988, the slope of the regression line = 1.14).
对25日龄大鼠单次注射孕马血清促性腺激素（50IU）后，卵巢组织的促卵泡激素（FSH）受体含量持续4天逐渐增加，随后呈下降趋势，而促黄体生成素（LH）受体含量在3天内保持不变，之后逐渐升高。基于这些事实，我们建立了一种放射受体测定系统，使用孕马血清促性腺激素注射3天后获得的卵巢匀浆3000转/分钟沉淀物作为受体制剂。解离标准曲线是用0.125 - 16纳克/管的美国国立卫生研究院糖尿病、消化和肾脏疾病研究所（NIAMDD）大鼠FSH I - 3获得的。纯化制剂NIAMDD大鼠LH I - 4和NIAMDD大鼠促甲状腺激素（TSH）I - 4仅在高浓度时影响结合，这可能是由于FSH污染所致。当用该系统检测从处于各种生理状态的大鼠获得的垂体前叶匀浆时，批内变异系数和批间变异系数分别为7.5%和13.7%，测定值与放射免疫测定获得的值高度相关（r = 0.988，回归线斜率 = 1.14）。
