Minegishi T, Igarashi M, Wakabayashi K
Endocrinol Jpn. 1982 Apr;29(2):233-40. doi: 10.1507/endocrj1954.29.233.
We established a sensitive RRA system for human FSH, employing PMS-treated immature rat ovary. The dissociation constant of the binding of the receptor preparation to NIAMDD human FSH-2 was 1.15 x 10(-10) M. The standard curve was obtained with 0.2-25.6 ng/tube of NIAMDD hFSH-2. Purified hLH, hTSH, and HCG had no significant effect on the binding. When the anterior pituitary homogenates obtained from humans were assayed by this system, the intra-assay and inter-assay coefficients of variation were 11.9% and 13.4% respectively, and the assay values correlated well with those obtained by RIA. This assay is applicable for the measurement of FSH in serum, when the non-specific inhibitor effects of serum are compensated for by the addition of merthiolate and when FSH-free serum is used instead of the buffer for the standard curve. The intra-assay and inter-assay coefficients of variation were 9.31% and 19.7% respectively. The assay values correlated with those obtained by RIA under the same physiological state. The ratio of the assay values RRA/RIA, was dependent upon the physiological state, e.g. 6.29 in men, 3.84 and 4.18 in women at follicular and luteal phase respectively and 2.40 in menopausal women. During the menstrual cycle, our results showed that the value of RRA/RIA derived from serum did not change significantly.
我们利用经孕马血清促性腺激素（PMS）处理的未成熟大鼠卵巢，建立了一种用于检测人促卵泡生成素（FSH）的灵敏放射受体分析法（RRA）。受体制剂与美国国立卫生研究院糖尿病、消化和肾脏疾病研究所（NIAMDD）人促卵泡生成素-2（hFSH-2）结合的解离常数为1.15×10⁻¹⁰ M。标准曲线是用0.2 - 25.6 ng/管的NIAMDD hFSH-2绘制的。纯化的人促黄体生成素（hLH）、人促甲状腺激素（hTSH）和人绒毛膜促性腺激素（HCG）对结合无显著影响。当用该系统检测从人垂体前叶获得的匀浆时，批内和批间变异系数分别为11.9%和13.4%，并且分析值与放射免疫分析法（RIA）获得的值相关性良好。当通过添加硫柳汞补偿血清的非特异性抑制作用，并使用无FSH血清代替缓冲液绘制标准曲线时，该分析法适用于血清中FSH的测定。批内和批间变异系数分别为9.31%和19.7%。在相同生理状态下，分析值与RIA获得的值相关。分析值的RRA/RIA比值取决于生理状态，例如男性为6.29，女性卵泡期和黄体期分别为3.84和4.18，绝经后女性为2.40。在月经周期中，我们的结果表明，血清来源的RRA/RIA值没有显著变化。
