体外培养的人外周血淋巴细胞染色体畸变的起源。I. 用粗糙脉孢菌核酸内切酶和聚乙二醇进行的实验。
On the origin of chromosomal aberrations in human peripheral lymphocytes in vitro. I. Experiments with Neurospora endonuclease and polyethylene glycol.
作者信息
Nowak C, Obe G
出版信息
Hum Genet. 1984;66(4):335-43. doi: 10.1007/BF00287637.
PMID:6327498
Abstract
Post-treatment of mutagen-treated human peripheral lymphocytes with a single-strand specific endonuclease from Neurospora crassa leads to a significant elevation of the rate of structural chromosomal aberrations. Our results indicate that DNA double-strand breaks (DSB) are ultimate lesions for the formation of chromosomal aberrations in the G1 and G2 phase of the cell cycle and probably also in the S-phase. Post-treatment of X-irradiated G2 cells with polyethylene glycol (PEG) leads to an elevation of the frequencies of chromatid type aberrations. This result is taken as an indication that nucleases from PEG-damaged lysosomes transform lesions in X-ray damaged chromosomes to DSB. With respect to the origin of chromosomal aberrations, our results are in favour of the breakage and reunion hypothesis of K. Sax , and not of Revell 's exchange hypothesis.
摘要
用来自粗糙脉孢菌的单链特异性核酸内切酶对经诱变处理的人外周血淋巴细胞进行后处理，会导致染色体结构畸变率显著升高。我们的结果表明，DNA双链断裂（DSB）是细胞周期G1期和G2期以及可能在S期形成染色体畸变的最终损伤。用聚乙二醇（PEG）对X射线照射的G2期细胞进行后处理，会导致染色单体型畸变频率升高。这一结果表明，来自PEG损伤溶酶体的核酸酶将X射线损伤染色体中的损伤转化为DSB。关于染色体畸变的起源，我们的结果支持K. Sax的断裂重接假说，而不支持Revell的交换假说。
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