Snodgrass D R, Fahey K J, Wells P W, Campbell I, Whitelaw A
Infect Immun. 1980 May;28(2):344-9. doi: 10.1128/iai.28.2.344-349.1980.
Ten heifers were inoculated on two occasions with an inactivated preparation of tissue culture-grown calf rotavirus, and a further ten heifers received a placebo vaccine. Serum anti-rotavirus antibody titers were significantly increased throughout pregnancy in the vaccinated group. After calving, the mean neutralizing antibody titer of colostral whey in control cows was 100, associated with immunoglobulins A and G1. No antibody was detected in the milk of these cows after the 4th day postpartum. The colostral whey from the vaccinated cows had a mean antibody titer of 20,452; 28 days after calving, the mean milk antibody titer was 320, associated mainly with immunoglobulin G1. Calves were challenged with a large oral inoculum of calf rotavirus at the 7th day of age. There was significant lengthening of the incubation and prepatent periods in calves born to vaccinated dams, but rotavirus-associated diarrhea of equal severity occurred in both groups. Evidence is presented which suggests that rotavirus antibody in milk can protect against a smaller challenge dose. Maternal immunization against rotavirus may be a practical proposition.
十头小母牛分两次接种了组织培养生长的小牛轮状病毒灭活制剂，另有十头小母牛接种了安慰剂疫苗。在整个孕期，接种组的血清抗轮状病毒抗体滴度显著升高。产犊后，对照母牛初乳乳清的平均中和抗体滴度为100，与免疫球蛋白A和G1相关。产后第4天之后，这些母牛的乳汁中未检测到抗体。接种疫苗的母牛初乳乳清的平均抗体滴度为20452；产犊后28天，乳汁平均抗体滴度为320，主要与免疫球蛋白G1相关。小牛在7日龄时经口接种大量小牛轮状病毒。接种疫苗的母牛所生小牛的潜伏期和潜隐期显著延长，但两组中与轮状病毒相关的腹泻严重程度相同。有证据表明，乳汁中的轮状病毒抗体可抵御较小剂量的攻击。对轮状病毒进行母体免疫可能是可行的方案。
