Middleton P J, Petric M, Hewitt C M, Szymanski M T, Tam J S
J Clin Pathol. 1976 Mar;29(3):191-7. doi: 10.1136/jcp.29.3.191.
A moderatley sensitive, rapid, and economical test scheme for the detection of infantile gastroenteritis virus (IGV) in stool or antibody in serum has been developed and evaluated. The test scheme with minor modifications was an adaptation of a counter-immunoelectro-osmophoresis system we once used for the detection of hepatitis B antigen. Large numbers of stool samples may be screened during half a working day for the presence of IGV using reference antiserum to IGV prepared in guinea-pigs. Serological studies of a diagnostic but not epidemiological nature may also be performed with equal facility by this same test scheme using highly purified IGV antigen derived from stool.
已开发并评估了一种用于检测粪便中婴儿肠胃炎病毒（IGV）或血清中抗体的中度灵敏、快速且经济的检测方案。经过微小修改的该检测方案是我们曾经用于检测乙型肝炎抗原的对流免疫电渗析系统的一种改编。使用在豚鼠中制备的IGV参考抗血清，可在半个工作日内对大量粪便样本进行筛查，以检测IGV的存在。使用源自粪便的高度纯化的IGV抗原，通过相同的检测方案，也可同样方便地进行具有诊断而非流行病学性质的血清学研究。
