Ciechanover A, Elias S, Heller H, Ferber S, Hershko A
J Biol Chem. 1980 Aug 25;255(16):7525-8.
A heat-stable polypeptide from rabbit reticulocytes has been previously shown to be required for ATP-dependent protein breakdown (Ciechanover, A., Hod, Y., and Hershko, A. (1978) Biochem. Biophys. Res Commun. 81, 1100-1105) and to form covalent conjugates with proteins in an ATP-requiring reaction (Ciechanover, A., Heller, H., Elias, S., Haas, A.L., and Hershko, A. (1980) Proc. Natl. Acad. Sci. U.S.A. 77, 1365-1368). We now describe its purification, characterization, and tissue distribution. Its presence in erythrocytes at high level makes this a possible preferred source in the future.
先前已表明，兔网织红细胞中的一种热稳定多肽是ATP依赖性蛋白质分解所必需的（Ciechanover, A., Hod, Y., and Hershko, A. (1978) Biochem. Biophys. Res Commun. 81, 1100 - 1105），并且在需要ATP的反应中与蛋白质形成共价缀合物（Ciechanover, A., Heller, H., Elias, S., Haas, A.L., and Hershko, A. (1980) Proc. Natl. Acad. Sci. U.S.A. 77, 1365 - 1368）。我们现在描述其纯化、特性鉴定及组织分布情况。它在红细胞中的高含量使其有可能成为未来首选的来源。
