Rubio C A, Lagerlöf B, Kock Y, Söderberg G, Thomassen P
J Natl Cancer Inst. 1978 Mar;60(3):523-6. doi: 10.1093/jnci/60.3.523.
Autoradiograms of histologic slides of 58 human cone specimens with dysplasia and carcinoma in situ were analyzed after tissue samples were incubated with labeled RNA precursors. Both the vertical and lateral distributions of labeled cells were rather uniform in the major part of the epithelium, which suggested that the tissue remained metabolically active during incubation. Only the uppermost epithelial cells in heavily labeled areas were devitalized as deduced by the morphologic appearance of the cells, the absence of labeling in the cells, the trypan blue exclusion test, and the trypsin digestion test. The viability of large epithelial areas suggested that the previously reported focal distribution of proliferating and nonproliferating areas in the cervical epithelium is a genuine phenomenon and not the result of focal epithelial devitalization acquired during incubation.
在将组织样本与标记的RNA前体一起孵育后，对58例有发育异常和原位癌的人锥体标本的组织学切片进行了放射自显影片分析。标记细胞的垂直和横向分布在上皮的大部分区域相当均匀，这表明组织在孵育过程中仍保持代谢活性。根据细胞的形态外观、细胞中标记物的缺失、台盼蓝排斥试验和胰蛋白酶消化试验推断，只有标记严重区域的最上层上皮细胞失去了活力。大面积上皮区域的活力表明，先前报道的宫颈上皮中增殖区和非增殖区的局灶性分布是一种真实现象，而不是孵育过程中获得的局灶性上皮失活的结果。
