Petersen B L, Petersen C L, Braendstrup O, Mouritsen S, Engel A M, Svane I M, Werdelin O
Department of Pathology, KAS Glostrup, Denmark.
APMIS. 1993 Jul;101(7):529-36. doi: 10.1111/j.1699-0463.1993.tb00142.x.
Many human tumors express low amounts of HLA class I molecules relative to the normal cells from which they are derived. From experimental work it is clear that the malignant behavior of a tumor cell may depend on its MHC class I expression. Therefore, it is of obvious interest to study the HLA class I expression of human tumors in their various stages. We have studied the HLA class I expression by the cells in premalignant epithelial lesions and invasive carcinoma of the bladder and uterine cervix using immunoperoxidase staining for beta 2-microglobulin of paraffin-embedded tissue. We here assume that beta 2-microglobulin expression by malignant and premalignant cells equals HLA class I expression. Thirty-two of the 36 invasive tumors expressed less overall beta 2-microglobulin than cells from the normal epithelium. In contrast, approximately two-thirds of 34 premalignant bladder epithelia and 47 premalignant cervix epithelia displayed higher overall beta 2-microglobulin expression than the normal epithelium. Thus, a systematic large-scale elimination of HLA class I high-expressing tumor cell variants may take place only after the tumor penetrates the basement membrane.
与产生它们的正常细胞相比，许多人类肿瘤表达的HLA I类分子数量较少。从实验工作中可以清楚地看出，肿瘤细胞的恶性行为可能取决于其MHC I类分子的表达。因此，研究人类肿瘤在不同阶段的HLA I类分子表达显然具有重要意义。我们使用免疫过氧化物酶法对石蜡包埋组织中的β2-微球蛋白进行染色，研究了膀胱和子宫颈癌前上皮病变及浸润性癌中细胞的HLA I类分子表达。我们在此假设恶性和癌前细胞的β2-微球蛋白表达等同于HLA I类分子表达。36例浸润性肿瘤中有32例总体β2-微球蛋白表达低于正常上皮细胞。相比之下，34例癌前膀胱上皮和47例癌前子宫颈上皮中约三分之二的总体β2-微球蛋白表达高于正常上皮。因此，只有在肿瘤穿透基底膜后，才可能系统性地大规模清除高表达HLA I类分子的肿瘤细胞变体。
