Leranth C, Williams T H, Chretien M, Palkovits M
Cell Tissue Res. 1980;210(1):11-9. doi: 10.1007/BF00232137.
Fine structural localization of an ACTH-like substance was obtained in neurons of the rat arcuate nucleus using immuno-electron microscopy, whereas it could not be confirmed that ACTH-containing cell bodies are present in the supraoptic nucleus. The immunoreactive cells of the arcuate nucleus appeared to be more numerous than the unreactive neurons. Immunostaining was carried out before embedding in resin. Empty vesicles of irregular shape were found in dendrites of immunoreactive arcuate neurons, but their significance and nature remain enigmatic. The reaction product was distributed uniformly throughout the cytoplasm of the ACTH-positive cells, except that the mitochondria, rough endoplasmic reticulum and Golgi vesicles and cisternae were devoid of PAP molecules. This distribution differed from the localization reported in ACTH-secreting cells of the rat anterior pituitary, where the reaction product was found in the rough endoplasmic reticulum and Golgi complex as well as in secretory granules.
利用免疫电子显微镜技术在大鼠弓状核的神经元中获得了促肾上腺皮质激素（ACTH）样物质的精细结构定位，而在视上核中未能证实存在含ACTH的细胞体。弓状核的免疫反应性细胞似乎比无反应性神经元数量更多。在包埋于树脂之前进行免疫染色。在免疫反应性弓状神经元的树突中发现了形状不规则的空泡，但其意义和性质仍不清楚。反应产物均匀分布于ACTH阳性细胞的整个细胞质中，除了线粒体、粗面内质网、高尔基体小泡和扁平囊泡中没有过氧化物酶抗过氧化物酶（PAP）分子。这种分布与大鼠垂体前叶分泌ACTH细胞中报道的定位不同，在垂体前叶分泌ACTH细胞中，反应产物存在于粗面内质网、高尔基体复合体以及分泌颗粒中。
