Miyazaki T, Mizukoshi H, Araki Y, Shimizu N
Endocrinol Jpn. 1980 Apr;27(2):175-82. doi: 10.1507/endocrj1954.27.175.
Urinary metabolites of [6,7-3H]-estriol-3-glucosiduronate and of [6, 7-3H]-estriol in intact female rabbits were analyzed. The separation of urinary metabolites was performed by countercurrent distribution followed by DEAE-Sephadex A-25 column chromatography. Each conjugate was then hydrolyzed with the enzymes and the aglycone thus liberated was identified. In either case, major urinary metabolites were found to be diconjugates, a considerable part of which was glucosiduronate-N-acetylglucosaminide of 17-epiestriol. In addition, estriol-16-glucosiduronate or monoglucosiduronate of 17-epiestriol was identified as a minor urinary metabolite of [6,7-3H]-estriol. From these results, it was concluded that the greater part of the estriol-3-glucosiduronate was converted to diconjugates and that estriol-3-glucosiduronate was probably an intermediate metabolite in the conversion pathway from estriol to diconjugates in this species.
对完整雌性家兔体内[6,7-³H]-雌三醇-3-葡萄糖醛酸苷和[6,7-³H]-雌三醇的尿液代谢产物进行了分析。尿液代谢产物的分离通过逆流分配法，随后进行DEAE-葡聚糖A-25柱色谱法。然后用酶将每种共轭物水解，并鉴定由此释放的苷元。在这两种情况下，主要的尿液代谢产物均为双共轭物，其中相当一部分是17-表雌三醇的葡萄糖醛酸苷-N-乙酰氨基葡萄糖苷。此外，雌三醇-16-葡萄糖醛酸苷或17-表雌三醇的单葡萄糖醛酸苷被鉴定为[6,7-³H]-雌三醇的次要尿液代谢产物。从这些结果可以得出结论，雌三醇-3-葡萄糖醛酸苷的大部分转化为双共轭物，并且雌三醇-3-葡萄糖醛酸苷可能是该物种中雌三醇向双共轭物转化途径中的中间代谢产物。
