Davis J C
Am J Med Technol. 1976 Nov;42(11):417-23.
A new Gram stain method for blood cultures was adapted from a method for tissue sections. This was accomplished by comparing various modifications of the latter method on positive smears prepared from a 24-hour negative blood culture seeded with Staphylococcus aureus and Escherichia coli. A blind study was then conducted using the new method and Hucker's method on 24-hour and 72-hour blood cultures as well as a limited number of other body fluids and tissues. Of the 35 positive blood cultures detected by the new method, the Hucker method failed to detect 14 positive specimens (40 per cent) upon initial examination. Ten additional cultures were negative using both stains, but were positive on subculture at three days. The 18 additional cultures positive after ten days were detected using only the Hucker stain.
一种用于血培养的新型革兰氏染色方法是从一种用于组织切片的方法改编而来。这是通过在接种了金黄色葡萄球菌和大肠杆菌的24小时阴性血培养制备的阳性涂片上比较后一种方法的各种改良方法来实现的。然后，使用这种新方法和哈克（Hucker）方法对24小时和72小时的血培养以及有限数量的其他体液和组织进行了一项盲法研究。在通过新方法检测出的35例阳性血培养中，哈克方法在初次检查时未能检测出14例阳性标本（40%）。另外有10份培养物两种染色方法均显示为阴性，但在三天的传代培养后呈阳性。仅使用哈克染色法检测出另外18份在十天后呈阳性的培养物。
