LaRochelle F T, North W G, Stern P
Pflugers Arch. 1980 Aug;387(1):79-81. doi: 10.1007/BF00580849.
Small columns packed with octadecasilyl-silica were used to extract arginine-vasopressin in femtomole amounts from biological fluids for radioimmunoassay. This method is effective in isolating the peptide from substances in plasma and serum which interfere with its quantitation. Consistent and reproducible results and recoveries > 80% were obtained with this procedure. High pressure liquid chromatography and serial dilution of extracted samples confirm the identity of the extracted product.
填充有十八烷基硅烷硅胶的小柱用于从生物流体中提取飞摩尔量的精氨酸加压素，以进行放射免疫测定。该方法可有效从血浆和血清中干扰其定量的物质中分离出该肽。采用此程序可获得一致且可重复的结果，回收率>80%。高压液相色谱法和对提取样品的系列稀释证实了提取产物的身份。
