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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Clin Sci Mol Med. 1976 Dec;51(6):525-36. doi: 10.1042/cs0510525.

Rabbit antisera against arginine-vasopressin (AVP) were evaluated for sensitivity and specificity in a radioimmunoassay based on the extraction of AVP from plasma and urine on to Florisil. 2. Comparison of the immunoreactivity of AVP with analogues showed that one antiserum (R2) reacted principally with the hexapeptide ring and another (R4) bound to the tripeptide tail and was reactive with some reduction and hydrolytic products of the native peptide. 3. The minimum amount of AVP measurable in the radioimmunoassay was 1 pg. The extraction of AVP from plasma and urine gave a recovery of 93 per cent (sd 5 per cent). A plasma sample repeatedly assayed with R2 gave a value of 1.4 ng/1 (sd 0.2, n=12). 4. The antiserum specific for the hexapeptide ring (R2) showed that in normal subjects AVP concentration ranged from 3.2 +/- 2.52 ng/1 after dehydration to 0.16 +/- 0.1 ng/1 after water loading.

基于从血浆和尿液中提取精氨酸加压素（AVP）至弗罗里硅土上，对兔抗精氨酸加压素抗血清在放射免疫分析中的敏感性和特异性进行了评估。2. AVP与类似物免疫反应性的比较表明，一种抗血清（R2）主要与六肽环反应，另一种（R4）与三肽尾结合，并与天然肽的一些还原和水解产物反应。3. 放射免疫分析中可测量的AVP最小量为1皮克。从血浆和尿液中提取AVP的回收率为93%（标准差5%）。用R2反复检测的一份血浆样本的值为1.4纳克/升（标准差0.2，n = 12）。4. 对六肽环特异的抗血清（R2）表明，在正常受试者中，AVP浓度在脱水后为3.2±2.52纳克/升，在饮水后为0.16±0.1纳克/升。

Clin Sci Mol Med. 1976 Dec;51(6):525-36. doi: 10.1042/cs0510525.

Rabbit antisera against arginine-vasopressin (AVP) were evaluated for sensitivity and specificity in a radioimmunoassay based on the extraction of AVP from plasma and urine on to Florisil. 2. Comparison of the immunoreactivity of AVP with analogues showed that one antiserum (R2) reacted principally with the hexapeptide ring and another (R4) bound to the tripeptide tail and was reactive with some reduction and hydrolytic products of the native peptide. 3. The minimum amount of AVP measurable in the radioimmunoassay was 1 pg. The extraction of AVP from plasma and urine gave a recovery of 93 per cent (sd 5 per cent). A plasma sample repeatedly assayed with R2 gave a value of 1.4 ng/1 (sd 0.2, n=12). 4. The antiserum specific for the hexapeptide ring (R2) showed that in normal subjects AVP concentration ranged from 3.2 +/- 2.52 ng/1 after dehydration to 0.16 +/- 0.1 ng/1 after water loading.

基于从血浆和尿液中提取精氨酸加压素（AVP）至弗罗里硅土上，对兔抗精氨酸加压素抗血清在放射免疫分析中的敏感性和特异性进行了评估。2. AVP与类似物免疫反应性的比较表明，一种抗血清（R2）主要与六肽环反应，另一种（R4）与三肽尾结合，并与天然肽的一些还原和水解产物反应。3. 放射免疫分析中可测量的AVP最小量为1皮克。从血浆和尿液中提取AVP的回收率为93%（标准差5%）。用R2反复检测的一份血浆样本的值为1.4纳克/升（标准差0.2，n = 12）。4. 对六肽环特异的抗血清（R2）表明，在正常受试者中，AVP浓度在脱水后为3.2±2.52纳克/升，在饮水后为0.16±0.1纳克/升。