人体血浆血管加压素的免疫测定
Immunoassay of plasma vasopressin in man.
作者信息
Robertson G L, Klein L A, Roth J, Gorden P
出版信息
Proc Natl Acad Sci U S A. 1970 Aug;66(4):1298-305. doi: 10.1073/pnas.66.4.1298.
Abstract
A radioimmunoassay for arginine vasopressin has been developed that is sensitive to the hormone at 1 pg/ml. When plasma was filtered on G-25 Sephadex, immunoreactivity was detected in three regions. Endogenous arginine vasopressin was the third and smallest of the peaks and was recovered just after the salt. By combining gel filtration with immunoassay, we have made precise measurements of arginine vasopressin in a few milliliters of plasma.
摘要
已开发出一种对精氨酸加压素敏感的放射免疫测定法，其对该激素的检测灵敏度为1皮克/毫升。当血浆在G-25葡聚糖凝胶上过滤时，在三个区域检测到免疫反应性。内源性精氨酸加压素是第三个也是最小的峰，在盐分之后被回收。通过将凝胶过滤与免疫测定相结合，我们已能够对几毫升血浆中的精氨酸加压素进行精确测量。
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