抗补体免疫荧光试验，该试验使用分离的成纤维细胞核来检测抗人巨细胞病毒抗体。
Anticomplement immunofluorescence test that uses isolated fibroblast nuclei for detection of antibodies to human cytomegalovirus.
作者信息
Mintz L, Miner R C, Yeager A S
出版信息
J Clin Microbiol. 1980 Oct;12(4):562-5. doi: 10.1128/jcm.12.4.562-565.1980.
Abstract
Cytomegalovirus antibodies were measured in human sera by a nuclear anticomplement immunofluorescence test that used as antigen the isolated nucleic of virus-infected fibroblasts cells lysed in distilled water. The method exhibited less nonspecific fluorescence than either a conventional whole-cell anticomplement immunofluorescence test or an indirect fluorescent antibody test applied to the same isolated nuclear substrate. The assay detected 97.5% of 40 antibody-positive sera, compared with 92.5 and 90% detection rates by indirect hemagglutination and complement fixation, respectively. In addition, antibody titers obtained by this technique were significantly higher than those obtained by either indirect hemagglutination (P < 0.02) or complement fixation with a glycine-extract antigen (P < 0.001).
摘要
采用核抗补体免疫荧光试验检测人血清中的巨细胞病毒抗体，该试验以在蒸馏水中裂解的病毒感染成纤维细胞的分离核酸作为抗原。与应用于相同分离核底物的传统全细胞抗补体免疫荧光试验或间接荧光抗体试验相比，该方法显示出较少的非特异性荧光。该检测法在40份抗体阳性血清中检测出97.5%，而间接血凝试验和补体结合试验的检测率分别为92.5%和90%。此外，通过该技术获得的抗体滴度显著高于通过间接血凝试验（P < 0.02）或用甘氨酸提取物抗原进行补体结合试验获得的抗体滴度（P < 0.001）。
相似文献
1
Anticomplement immunofluorescence test that uses isolated fibroblast nuclei for detection of antibodies to human cytomegalovirus.抗补体免疫荧光试验，该试验使用分离的成纤维细胞核来检测抗人巨细胞病毒抗体。
J Clin Microbiol. 1980 Oct;12(4):562-5. doi: 10.1128/jcm.12.4.562-565.1980.
2
Use of isolated nuclei in the indirect fluorescent-antibody test for human cytomegalovirus infection: comparison with microneutralization, anticomplement, and conventional indirect fluorescent-antibody assays.人巨细胞病毒感染间接荧光抗体试验中分离细胞核的应用：与微量中和试验、抗补体试验及传统间接荧光抗体试验的比较
J Clin Microbiol. 1978 May;7(5):486-9. doi: 10.1128/jcm.7.5.486-489.1978.
3
Anti-complement immunofluorescence test for antibodies to human cytomegalovirus.人巨细胞病毒抗体的抗补体免疫荧光试验
J Clin Microbiol. 1977 Dec;6(6):627-32. doi: 10.1128/jcm.6.6.627-632.1977.
4
Immunity to human cytomegalovirus measured and compared by complement fixation, indirect fluorescent-antibody, indirect hemagglutination, and enzyme-linked immunosorbent assays.通过补体结合试验、间接荧光抗体试验、间接血凝试验和酶联免疫吸附测定法检测并比较人巨细胞病毒的免疫力。
J Clin Microbiol. 1984 Feb;19(2):147-52. doi: 10.1128/jcm.19.2.147-152.1984.
5
Comparison of enzyme-linked immunosorbent assay, radioimmunoassay, complement fixation, anticomplement immunofluorescence and passive haemagglutination techniques for detecting cytomegalovirus IgG antibody.酶联免疫吸附测定、放射免疫测定、补体结合试验、抗补体免疫荧光法及被动血凝技术检测巨细胞病毒IgG抗体的比较
J Clin Pathol. 1982 Dec;35(12):1345-8. doi: 10.1136/jcp.35.12.1345.
6
Detection of antibodies to cytomegalovirus by an improved counterimmunoelectrophoretic procedure.通过改进的对流免疫电泳法检测巨细胞病毒抗体。
J Med Virol. 1982;10(1):17-24. doi: 10.1002/jmv.1890100104.
7
A comparison of complement fixation, indirect immunofluorescence for viral late antigens, and anti-complement immunofluorescence tests for the detection of cytomegalovirus specific serum antibodies.补体结合试验、病毒晚期抗原间接免疫荧光试验以及抗补体免疫荧光试验用于检测巨细胞病毒特异性血清抗体的比较。
J Clin Pathol. 1978 Sep;31(9):827-31. doi: 10.1136/jcp.31.9.827.
8
Evaluation of the FIAX test for the detection of antibodies to herpes simplex virus and cytomegalovirus.用于检测单纯疱疹病毒和巨细胞病毒抗体的FIAX检测法评估
Am J Clin Pathol. 1981 Mar;75(3):387-90. doi: 10.1093/ajcp/75.3.387.
9
Comparison of five methods of cytomegalovirus antibody screening of blood donors.献血者巨细胞病毒抗体筛查五种方法的比较。
J Clin Microbiol. 1983 Dec;18(6):1296-300. doi: 10.1128/jcm.18.6.1296-1300.1983.
10
Comparison of six methods for the detection of antibody to cytomegalovirus.六种检测巨细胞病毒抗体方法的比较
J Clin Microbiol. 1985 Dec;22(6):1014-9. doi: 10.1128/jcm.22.6.1014-1019.1985.
引用本文的文献
1
Antibodies to cytomegalovirus-induced pre-early nuclear antigen in the anticomplement-immunofluorescent test in comparison to IgG and IgM antibodies in the indirect and direct enzyme-linked immunosorbent assay in diagnosing cytomegalovirus infections.在诊断巨细胞病毒感染时，与间接和直接酶联免疫吸附测定中的IgG和IgM抗体相比，抗补体免疫荧光试验中针对巨细胞病毒诱导的早期核前抗原的抗体。
Arch Virol. 1984;80(4):305-13. doi: 10.1007/BF01311221.
2
Evaluation of murine cytomegalovirus antibody detection by serological techniques.通过血清学技术评估小鼠巨细胞病毒抗体检测
J Clin Microbiol. 1983 Oct;18(4):753-8. doi: 10.1128/jcm.18.4.753-758.1983.
3
The importance of primary cytomegalovirus infection in childhood cancer.原发性巨细胞病毒感染在儿童癌症中的重要性。
Eur J Pediatr. 1987 Jan;146(1):34-7. doi: 10.1007/BF00647279.
4
Comparison of six methods for the detection of antibody to cytomegalovirus.六种检测巨细胞病毒抗体方法的比较
J Clin Microbiol. 1985 Dec;22(6):1014-9. doi: 10.1128/jcm.22.6.1014-1019.1985.
5
Detection of human T-cell lymphotropic virus type III-related antigens and anti-human T-cell lymphotropic virus type III antibodies by anticomplementary immunofluorescence.用抗补体免疫荧光法检测人III型嗜T细胞病毒相关抗原及抗人III型嗜T细胞病毒抗体
J Clin Microbiol. 1986 Jun;23(6):1072-7. doi: 10.1128/jcm.23.6.1072-1077.1986.
6
Nonpulmonary manifestations of cytomegalovirus infection in immunocompromised patients.免疫功能低下患者巨细胞病毒感染的非肺部表现。
Clin Microbiol Rev. 1992 Apr;5(2):204-10. doi: 10.1128/CMR.5.2.204.
本文引用的文献
1
Comparative evaluation of commercial precipitating antisera against human IgM and IgM.针对人IgM和IgM的市售沉淀抗血清的比较评估。
J Lab Clin Med. 1970 Dec;76(6):949-60.
2
Transfusion-acquired cytomegalovirus infection in newborn infants.新生儿输血获得性巨细胞病毒感染
Am J Dis Child. 1974 Oct;128(4):478-83. doi: 10.1001/archpedi.1974.02110290048008.
3
A serologic study of cytomegalovirus infections associated with blood transfusions.一项关于与输血相关的巨细胞病毒感染的血清学研究。
N Engl J Med. 1971 May 20;284(20):1125-31. doi: 10.1056/NEJM197105202842004.
4
Indirect hemagglutination test for detection of antibodies to Cytomegalovirus.用于检测巨细胞病毒抗体的间接血凝试验。
Appl Microbiol. 1971 Jan;21(1):84-9. doi: 10.1128/am.21.1.84-89.1971.
5
Evaluation of the immunological specificity of fluorescein-labelled anti-human IgM conjugates.荧光素标记的抗人 IgM 结合物的免疫特异性评估。
Immunology. 1972 Jul;23(1):7-12.
6
Cytomegalovirus pneumonia after human marrow transplantation.人类骨髓移植后的巨细胞病毒肺炎
Ann Intern Med. 1975 Feb;82(2):181-8. doi: 10.7326/0003-4819-82-2-181.
7
Improved indirect hemagglutination test for cytomegalovirus using human O erythrocytes in lysine.使用赖氨酸处理的人O型红细胞改进巨细胞病毒间接血凝试验。
J Clin Microbiol. 1979 Jul;10(1):64-8. doi: 10.1128/jcm.10.1.64-68.1979.
8
A comparison of complement fixation, indirect immunofluorescence for viral late antigens, and anti-complement immunofluorescence tests for the detection of cytomegalovirus specific serum antibodies.补体结合试验、病毒晚期抗原间接免疫荧光试验以及抗补体免疫荧光试验用于检测巨细胞病毒特异性血清抗体的比较。
J Clin Pathol. 1978 Sep;31(9):827-31. doi: 10.1136/jcp.31.9.827.
9
Analysis of antibody assay methods and classes of viral antibodies in serodiagnosis of cytomegalovirus infection.巨细胞病毒感染血清学诊断中抗体检测方法及病毒抗体类别的分析。
J Clin Microbiol. 1978 Aug;8(2):153-9. doi: 10.1128/jcm.8.2.153-159.1978.
10
Use of isolated nuclei in the indirect fluorescent-antibody test for human cytomegalovirus infection: comparison with microneutralization, anticomplement, and conventional indirect fluorescent-antibody assays.人巨细胞病毒感染间接荧光抗体试验中分离细胞核的应用：与微量中和试验、抗补体试验及传统间接荧光抗体试验的比较
J Clin Microbiol. 1978 May;7(5):486-9. doi: 10.1128/jcm.7.5.486-489.1978.
Zotero 插件