Garfinkel D J, Nester E W
J Bacteriol. 1980 Nov;144(2):732-43. doi: 10.1128/jb.144.2.732-743.1980.
Mutants of Agrobacterium tumefaciens which affect virulence or the ability to catabolize octopine were isolated after Tn5-induced mutagenesis. Of 8,900 colonies tested, 7 mutants with Tn5 insertions in a specific region of other Ti plasmid unable to catabolize octopine were isolated. Thirty-seven mutants affected in tumorigenesis resulted from insertions in the Ti plasmid and the Agrobacterium chromosome. Of these mutations, 12 were chromosomal and 25 mapped on the plasmid. Twenty-three mapped within a 20-megadalton region, which is distinct from the Ti plasmid sequences found stably integrated into the plant cell genome T-deoxyribonucleic acid). Included in these were mutants that were either a virulent or produced tumors with unusual morphologies. Three mutants contained insertions in the T-deoxyribonucleic acid. These three mutants incited tumors which synthesized octopine but had an altered morphology due to either extensive proliferation of shoots or roots from the tumor callus. Three additional mutants not caused by Tn5 contained mutations in the Ti plasmid.
在经Tn5诱变后，分离得到了影响根癌土壤杆菌毒力或章鱼碱分解代谢能力的突变体。在检测的8900个菌落中，分离出7个在其他Ti质粒特定区域有Tn5插入且无法分解章鱼碱的突变体。37个影响肿瘤发生的突变体是由Ti质粒和根癌土壤杆菌染色体中的插入引起的。在这些突变中，12个是染色体突变，25个定位在质粒上。23个定位在一个20兆道尔顿的区域内，该区域与稳定整合到植物细胞基因组T-脱氧核糖核酸中的Ti质粒序列不同。其中包括无毒或产生形态异常肿瘤的突变体。三个突变体在T-脱氧核糖核酸中有插入。这三个突变体诱发的肿瘤能合成章鱼碱，但由于肿瘤愈伤组织中芽或根的大量增殖而形态发生改变。另外三个非Tn5引起的突变体在Ti质粒中含有突变。
