Klapwijk P M, van Breukelen J, Korevaar K, Ooms G, Schilperoort R A
J Bacteriol. 1980 Jan;141(1):129-36. doi: 10.1128/jb.141.1.129-136.1980.
A transfer-deficient derivative of plasmid RP1-pMG1 was isolated after insertion of Mu cts62. The Tra- R plasmid was used to donate Tn904, encoding streptomycin resistance, to Ti plasmid pAL102 harbored by Agrobacterium tumefaciens Ach5. Under conditions promoting high Ti transfer frequencies, 155 strains were isolated in which the streptomycin marker coupled with Ti plasmid in further transfer experiments. These isolates represent stable insertions of Tn904 into the Ti plasmid. In addition, 19 strains were isolated in which the insertion of Tn904 was apparently unstable. The frequency of stable Tn904 transpositions was estimated to be 3 x 10(4-) per transferred Ti plasmid. Evidence was obtained that Tn904 readily may transpose from the Ti plasmid into the bacterial chromosome. The strains carrying Ti plasmids with stable insertions were characterized with respect to virulence, octopine degradation, octopine synthesis in induced tumors, and Ti plasmid transfer. Thirteen of the strains were found to be affected in tumor-inducing ability.
在插入Mu cts62后，分离得到了质粒RP1-pMG1的转移缺陷衍生物。利用该Tra- R质粒将编码链霉素抗性的Tn904转移至根癌土壤杆菌Ach5所携带的Ti质粒pAL102中。在促进Ti高转移频率的条件下，分离得到155株菌株，在进一步的转移实验中，链霉素标记与Ti质粒相连。这些分离株代表了Tn904稳定插入到Ti质粒中。此外，还分离得到19株菌株，其中Tn904的插入明显不稳定。稳定的Tn904转座频率估计为每转移一个Ti质粒3×10⁻⁴。有证据表明，Tn904很容易从Ti质粒转座到细菌染色体中。对携带稳定插入Ti质粒的菌株进行了毒力、章鱼碱降解、诱导肿瘤中章鱼碱合成以及Ti质粒转移等方面的特性分析。发现其中13株菌株的肿瘤诱导能力受到影响。
Zotero 插件