对内源性RNA肿瘤病毒的自身免疫。小鼠血清抗体水平的放射免疫沉淀测定。
Autogenous immunity to endogenous RNA tumor virus. Radioimmune precipitation assay of mouse serum antibody levels.
作者信息
Ihle J N, Yurconic M, Hanna M G
出版信息
J Exp Med. 1973 Jul 1;138(1):194-208. doi: 10.1084/jem.138.1.194.
Abstract
The radioimmune precipitation assay using (3)H-labeled AKR leukemia virus was applied to the detection and quantitation of natural serum antibodies directed against endogenous murine leukemia virus (MuLV) envelope antigens B6C3F(1) and BALB/c mice, which have low natural incidences of leukemia and lymphoma, and AKR mice, which have a high incidence, were used in this study. Sera from mice of various age groups were assayed. A marked difference in age-associated levels of the autogenous immune response to endogenous murine leukemia virus was detected, and the quantitative differences among these strains were inversely related to the incidence of lymphoma. The radioimmune precipitation test as applied was 500 times more sensitive than virus neutralization. That the reactions we have observed are specific is suggested by several lines of evidence, including the nonreactivity of normal hamster and absorbed rat serum, the positive reaction of absorbed rat anti-AKR serum, the inhibition of precipitation of labeled virus by purified unlabeled virus, and isopycnic gradient analysis of the reactive products.
摘要
采用³H标记的AKR白血病病毒进行放射免疫沉淀试验，以检测和定量针对内源性鼠白血病病毒（MuLV）包膜抗原的天然血清抗体。本研究使用了白血病和淋巴瘤自然发病率较低的B6C3F(1)和BALB/c小鼠，以及发病率较高的AKR小鼠。对不同年龄组小鼠的血清进行了检测。检测到对内源性鼠白血病病毒的自身免疫反应水平在年龄上存在显著差异，并且这些品系之间的定量差异与淋巴瘤的发病率呈负相关。所应用的放射免疫沉淀试验比病毒中和试验敏感500倍。包括正常仓鼠血清和吸收后的大鼠血清无反应性、吸收后的大鼠抗AKR血清呈阳性反应、纯化的未标记病毒抑制标记病毒的沉淀以及对反应产物进行等密度梯度分析等多条证据表明，我们观察到的反应具有特异性。
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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