Murad S, Sivarajah A, Pinnell S R
J Invest Dermatol. 1980 Nov;75(5):404-7. doi: 10.1111/1523-1747.ep12523660.
Prolyl and lysyl hydroxylase activities in cultures of human skin fibroblasts from fetal to 94-yr-old donors were measured. In contrast to earlier studies with whole skin, neither prolyl nor lysyl hydroxylase activity was found related to donor age. Prolyl hydroxylase activity increased 3- to 6-fold when cell extracts were incubated with ascorbate and other hydroxylation cofactors before assay. A similar increase in prolyl hydroxylase activity occurred when cells were incubated with ascorbate. Lysyl hydroxylase activity remained unaltered under these conditions.
测定了来自胎儿至94岁供体的人皮肤成纤维细胞培养物中的脯氨酰羟化酶和赖氨酰羟化酶活性。与早期对全皮的研究不同，未发现脯氨酰羟化酶和赖氨酰羟化酶活性与供体年龄有关。在测定前，当细胞提取物与抗坏血酸和其他羟化辅因子一起孵育时，脯氨酰羟化酶活性增加了3至6倍。当细胞与抗坏血酸一起孵育时，脯氨酰羟化酶活性也出现类似增加。在这些条件下，赖氨酰羟化酶活性保持不变。
