Easton A J, Clements J B
Nucleic Acids Res. 1980 Jun 25;8(12):2627-45. doi: 10.1093/nar/8.12.2627.
Nuclear and cytoplasmic virus RNAs, synthesized in cells infected with herpes simplex virus type 2 at early and late times post-infection, and in the continuous presence of the protein synthesis inhibitor cycloheximide (immediate early), have been analyzed by blot hybridization to virus DNA fragments generated by Bam HI and Eco RI restriction endonucleases. Polyadenylated immediate early mRNAs were separated on denaturing gels containing CH3HgOH giving three virus-specific mRNA bands of estimated sizes 4.7, 3.4 and 1.75 kb, and these have been mapped to five discrete regions of the genome. The polypeptides produced by in vitro translation of the HSV-2 immediate early mRNA's have been identified. Orientations of immediate early mRNA's on the virus genome have been determined by mapping cDNAs complementary to the 3'termini of the mRNAs.
在感染单纯疱疹病毒2型的细胞中，于感染后早期和晚期合成的核病毒RNA和细胞质病毒RNA，以及在蛋白质合成抑制剂环己酰亚胺持续存在的情况下（立即早期）合成的病毒RNA，已通过与由Bam HI和Eco RI限制性内切酶产生的病毒DNA片段进行印迹杂交分析。在含有CH3HgOH的变性凝胶上分离出聚腺苷酸化的立即早期mRNA，得到三条估计大小为4.7、3.4和1.75 kb的病毒特异性mRNA条带，并且已将这些条带定位到基因组的五个离散区域。已鉴定出由HSV-2立即早期mRNA体外翻译产生的多肽。通过绘制与mRNA的3'末端互补的cDNA图谱，确定了立即早期mRNA在病毒基因组上的方向。
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