Castleman H, Meredith R D, Erlanger B F
Nucleic Acids Res. 1980 Oct 10;8(19):4485-99. doi: 10.1093/nar/8.19.4485.
The RNA of a deleted strain (lacking Src gene) of an avian sarcoma virus (ASV) was examined by a newly developed immunoelectron microscopic procedure which uses anti-nucleotide antibodies as probes. After denaturation of the RNA and reaction with a high affinity, highly specific anti-7-methylguanosine-5'-phosphate (anti-pm 7G), 81% of 106 molecules examined were found to have antibody at one terminus, in agreement with the presence of a pm 7G cap in ASV-RNA. Hapten inhibition by pm 7G could be demonstrated. Experiments with anti-A and with anti-poly A gave results consistent with the known structure of ASV-RNA, in particular the presence of a 3' poly A tail. These studies illustrate the feasibility of using anti-nucleotide antibodies in a combined immunochemical and electron microscopic study of the fine structure of nucleic acids.
利用一种新开发的免疫电子显微镜技术，以抗核苷酸抗体作为探针，对一种禽肉瘤病毒（ASV）缺失株（缺乏Src基因）的RNA进行了检测。RNA变性后，与高亲和力、高特异性的抗7-甲基鸟苷-5'-磷酸（抗pm 7G）反应，在检测的106个分子中，81%在一端有抗体，这与ASV-RNA中存在pm 7G帽结构相符。pm 7G可证明有半抗原抑制作用。用抗A和抗多聚A进行的实验结果与ASV-RNA的已知结构一致，特别是存在3'多聚A尾。这些研究说明了在核酸精细结构的免疫化学和电子显微镜联合研究中使用抗核苷酸抗体的可行性。
