Bister K, Duesberg P H
Proc Natl Acad Sci U S A. 1979 Oct;76(10):5023-7. doi: 10.1073/pnas.76.10.5023.
Two major RNA species were found in several clonal isolates of avian erythroblastosis virus (AEV) and avian erythroblastosis-associated helper virus (AEAV) complexes: one of 8.7 kilobases (kb), the other of 5.5 kb. The 5.5-kb species was identified as AEV RNA because (i) it was absent from non-transforming AEAV isolated from the same virus complex, (ii) it was present in complexes of AEV and different helper viruses, and (iii) its structure is similar to that of avian acute leukemia viruses of the MC29 group. Molecular hybridization indicated that 54% of AEV RNA is specific and 46% is related to other viruses of the avian tumor virus group, particularly to AEAV, therefore termed group-specific. The genetic structure of AEV RNA was deduced by mapping oligonucleotides representing specific and group-specific sequences and by comparing the resulting map to maps of AEAV and of other avian tumor viruses derived previously. AEV RNA contains a gag gene-related, 5' group-specific section of 1 kb, an internal AEV-specific section of 3 kb unrelated to any other viral RNA tested, and a 3' group-specific section of 1.5 kb. The 5' section of AEV RNA is closely related to analogous 5' sections of the MC29 group viruses and is homologous with a 5' RNA section that is part of the gag gene of AEAV. The 3' section is also shared with AEAV RNA and includes a variant C-oligonucleotide near the 3' end that is different from the highly conserved counterparts of all other exogenous avian tumor viruses. By analogy with Rous sarcoma virus and the acute leukemia viruses of the MC29 group, the internal specific section of AEV RNA is thought to signal a third class of onc genes in avian tumor viruses. Comparisons with AEAV and the MC29 group viruses suggest that both the 5' gag-related and the internal specific RNA sections of AEV are necessary for onc gene function.
在禽成红细胞增多症病毒（AEV）和禽成红细胞增多症相关辅助病毒（AEAV）复合体的几个克隆分离株中发现了两种主要的RNA种类：一种为8.7千碱基（kb），另一种为5.5 kb。5.5 kb的种类被鉴定为AEV RNA，原因如下：（i）从同一病毒复合体中分离出的非转化性AEAV中不存在该种类；（ii）它存在于AEV与不同辅助病毒的复合体中；（iii）其结构与MC29组禽急性白血病病毒的结构相似。分子杂交表明，54%的AEV RNA是特异性的，46%与禽肿瘤病毒组的其他病毒相关，特别是与AEAV相关，因此被称为组特异性。通过对代表特异性和组特异性序列的寡核苷酸进行定位，并将所得图谱与先前推导的AEAV和其他禽肿瘤病毒的图谱进行比较，推导出了AEV RNA的遗传结构。AEV RNA包含一个与gag基因相关的1 kb的5'组特异性区段、一个3 kb的内部AEV特异性区段，该区段与所测试的任何其他病毒RNA均无关，以及一个1.5 kb的3'组特异性区段。AEV RNA的5'区段与MC29组病毒的类似5'区段密切相关，并且与作为AEAV gag基因一部分的5' RNA区段同源。3'区段也与AEAV RNA共有，并且在3'端附近包括一个变异的C寡核苷酸，该寡核苷酸与所有其他外源性禽肿瘤病毒的高度保守对应物不同。与劳氏肉瘤病毒和MC29组急性白血病病毒类似，AEV RNA的内部特异性区段被认为是禽肿瘤病毒中第三类癌基因的信号。与AEAV和MC29组病毒的比较表明，AEV的5' gag相关和内部特异性RNA区段对于癌基因功能都是必需的。
