Law W T, Ertingshausen G
Clin Chem. 1980 Dec;26(13):1874-7.
We report a novel reagent containing ferric perchlorate, perchloric acid, and polyoxyethylene (23) lauryl ether (Brij 35) with which the concentration of chloride in serum can be measured. We applied this reagent to use with a centrifugal analyzer (CentrifiChem 400) in a dynamic bichromatic procedure, resulting in broad linearity of the standard curve (0-180 mmol/L), short analysis time (1 min), and little interference from bilirubin, hemoglobin, turbidity, or bromide ions. The reagent is simple, contains no mercury, and the combination of low acid concentration and surfactant prevents serum protein precipitation. Precision is good (for x- = 93 mmol/L, CV = 1.55%), and results correlate well with those obtained by coulometry (r = 0.974).
我们报告了一种新型试剂，它含有高氯酸铁、高氯酸和聚氧乙烯（23）月桂醚（Brij 35），可用于测定血清中的氯化物浓度。我们将该试剂应用于离心分析仪（CentrifiChem 400）的动态双色法检测中，得到的标准曲线线性范围宽（0 - 180 mmol/L）、分析时间短（1分钟），并且胆红素、血红蛋白、浊度或溴离子的干扰小。该试剂简单，不含汞，低酸浓度与表面活性剂的组合可防止血清蛋白沉淀。精密度良好（对于x- = 93 mmol/L，CV = 1.55%），并且结果与库仑法所得结果具有良好的相关性（r = 0.974）。
