Shull B C, Lees H, Li P K
Clin Chem. 1980 Jan;26(1):22-5.
Oxyhemoglobin is the species of hemoglobin in erythrocyte hemolysates that inhibits the diazo reaction. Ferric hemoglobin derivatives and species with relatively low molecular mass do not interfere. Conversion of oxyhemoglobin to acid hematin under assay reaction conditions is associated with rapid destruction of bilirubin, which accounts for the diazo reaction error. The most probable mechanism for this destruction of bilirubin is an oxidative reaction involving H2O2, formed in the oxidation of hemoglobin, and acid hematin acting as a pseudoperoxidase. We could find no evidence for other mechanisms of interference such as spectral error or azobilirubin destruction. Addition of potassium iodide, 4.0 mmol/L final concentration in the reaction mixture, eliminates interference from hemoglobin added to give concentrations as great as 10 g/L. It also eliminated the effects of hemolysis in the method of Ertingshausen et al. (Clin. Chem. 19: 1366, 1973), in which ethylene glycol is used as the accelerator.
氧合血红蛋白是红细胞溶血产物中抑制重氮反应的血红蛋白种类。高铁血红蛋白衍生物和相对低分子量的种类不产生干扰。在测定反应条件下，氧合血红蛋白转化为酸性正铁血红素与胆红素的快速破坏有关，这导致了重氮反应误差。胆红素这种破坏最可能的机制是一种氧化反应，涉及血红蛋白氧化过程中形成的过氧化氢，以及酸性正铁血红素作为假过氧化物酶的作用。我们没有发现其他干扰机制的证据，如光谱误差或偶氮胆红素破坏。在反应混合物中加入终浓度为4.0 mmol/L的碘化钾，可消除加入浓度高达10 g/L的血红蛋白产生的干扰。它还消除了在Ertingshausen等人（《临床化学》19: 1366, 1973）的方法中溶血的影响，该方法中使用乙二醇作为促进剂。
