Boĭtsov A S, Rybchin V N
Genetika. 1980;16(5):777-82.
The phage hybrid phi80hy43 derived from a vegetative cross phi80cIhlambda x lambdacIc17 was constructed for discrimination phi80mono- and polylysogens. Molecular structure of this hybrid was established using heteroduplex analysis and restriction endonuclease EcoRI. It is found that the hybrid phi80hy43 represents a phage phi80 containing a foreign piece of DNA between genes cI and 0. The length of this piece of DNA comprises 0.7%, corresponding to the length of cy-cII region of th phage lambda. So it is believed that the hybrid phi80hy43 was formed due to insertion of the lambdacy region with the mutation c17 into phi80hlambda phage genome.
从营养杂交体phi80cIhlambda×lambdacIc17衍生而来的噬菌体杂交体phi80hy43被构建用于区分phi80单溶原菌和多溶原菌。利用异源双链分析和限制性内切酶EcoRI确定了该杂交体的分子结构。发现杂交体phi80hy43代表一种噬菌体phi80，其在cI和0基因之间含有一段外源DNA片段。这段DNA的长度占0.7%，相当于噬菌体lambda的cy-cII区域的长度。因此据信，杂交体phi80hy43是由于携带c17突变的lambda cy区域插入到phi80hlambda噬菌体基因组中而形成的。
