关于某些限制性核酸内切酶与甲基化底物使用的警示说明。
A cautionary note on the use of certain restriction endonucleases with methylated substrates.
作者信息
Backman K
出版信息
Gene. 1980 Oct;11(1-2):169-71. doi: 10.1016/0378-1119(80)90097-9.
PMID:6254852
Abstract
Methylation of adenine and cytosine residues in DNA isolated from common strains of Escherichia coli K-12 can render that DNA resistant to cleavage by certain restriction endonucleases at those sites at which the recognition sequence for such an endonuclease overlaps (but does not include) a sequence recognized by methylases specified by the dam or dcm gene.
摘要
从大肠杆菌K-12常见菌株中分离出的DNA中，腺嘌呤和胞嘧啶残基的甲基化可使该DNA在某些限制性内切酶的识别序列与由dam或dcm基因指定的甲基化酶识别的序列重叠（但不包括）的位点处，对这些限制性内切酶的切割产生抗性。
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