识别GpC的胞嘧啶-5- DNA甲基转移酶编码基因的克隆、特性分析及表达
Cloning, characterization and expression of the gene coding for a cytosine-5-DNA methyltransferase recognizing GpC.
作者信息
Xu M, Kladde M P, Van Etten J L, Simpson R T
机构信息
Department of Biochemistry and Molecular Biology and the Center for Gene Regulation, 308 Althouse Laboratory,The Pennsylvania State University, University Park, PA 16802-4500, USA.
出版信息
Nucleic Acids Res. 1998 Sep 1;26(17):3961-6. doi: 10.1093/nar/26.17.3961.
Abstract
A novel gene encoding a cytosine-5-DNA methyltransferase recognizing the dinucleotide GpC was cloned from Chlorella virus NYs-1 and expressed in both Escherichia coli and Saccharomyces cerevisiae . The gene was sequenced and a predicted polypeptide of 362 amino acids with a molecular weight of 41.903 kDa was identified. The protein contains several amino acid motifs with high similarity to those of other known 5-methylcytosine-forming methyltransferases. In addition, this enzyme, named M. Cvi PI, shares 66% identity and 76% similarity with M. Cvi JI, the only other cytosine-5-DNA methyltransferase cloned from a Chlorella virus. The short, frequently occurring recognition sequence of the new methyltransferase will be very useful for in vivo chromatin structure studies in both yeast and higher organisms.
摘要
从小球藻病毒 NYs-1 中克隆出一个编码识别二核苷酸 GpC 的胞嘧啶-5-DNA 甲基转移酶的新基因，并在大肠杆菌和酿酒酵母中表达。对该基因进行了测序，鉴定出一个预测的由 362 个氨基酸组成、分子量为 41.903 kDa 的多肽。该蛋白含有几个与其他已知的形成 5-甲基胞嘧啶的甲基转移酶高度相似的氨基酸基序。此外，这种名为 M. Cvi PI 的酶与从另一种小球藻病毒中克隆出的唯一的另一种胞嘧啶-5-DNA 甲基转移酶 M. Cvi JI 具有 66% 的同一性和 76% 的相似性。这种新甲基转移酶短且频繁出现的识别序列对于酵母和高等生物体内的染色质结构研究将非常有用。
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