从一组与翻译相关的基因中对大肠杆菌起始因子IF3的结构基因进行物理定位和克隆。

Physical localisation and cloning of the structural gene for E. coli initiation factor IF3 from a group of genes concerned with translation.

作者信息

Plumbridge J A, Springer M, Graffe M, Goursot R, Grunberg-Manago M

出版信息

Gene. 1980 Oct;11(1-2):33-42. doi: 10.1016/0378-1119(80)90084-0.

Abstract

The structural genes for translational initiation factor IF3, threonyl-tRNA synthetase (TRS), the two subunits of phenylalanyl-tRNA synthetase (PRS), and a 12 000 mol. wt. protein of unidentified function are carried by the lambda p2 transducing phage. The localization of these genes on a restriction map of the Escherichia coli DNA insert was achieved by deletion mapping. In addition a set of plasmids carrying fragments of the original phage was constructed and helped to confirm these assignments. One plasmid, containing a 3.3 kb PstI fragment inserted into pBR322, does not code for any of the synthetase genes but causes strains carrying it to overproduce IF3.

摘要

翻译起始因子IF3、苏氨酰-tRNA合成酶（TRS）、苯丙氨酰-tRNA合成酶（PRS）的两个亚基以及一种功能不明的12000分子量蛋白质的结构基因由λp2转导噬菌体携带。通过缺失作图实现了这些基因在大肠杆菌DNA插入片段限制酶切图谱上的定位。此外，构建了一组携带原始噬菌体片段的质粒，有助于证实这些定位。一种质粒含有插入pBR322的3.3 kb PstI片段，它不编码任何合成酶基因，但会使携带它的菌株过量产生IF3。