Kumari G L, Vohra S, Joshi L, Roy S
Horm Res. 1980;13(1):57-67. doi: 10.1159/000179271.
Aqueous extracts of frozen human corpora lutea were tested for the presence of an inhibitor of luteinizing hormone-receptor site binding (LHRBI) and for the subsequent effect on the stimulatory response of luteinizing hormone (LH) on progesterone synthesis by sheep ovarian cells. In the presence of human corpus luteum extract of normal menstrual cycle (30,000-g supernatant), the binding of 125I human chorionic gonadotrophin (hCG) to granulosa and luteal cells of sheep ovaries was markedly reduced, but the ability of rat testicular LH receptors to bind labelled hCG was less affected. However, extracts of corpora lutea of the first trimester of pregnancy appeared to be less inhibitory on the binding of LH/hCG to ovarian cells and had no effect on the binding of rat testicular cells compared to those of normal menstrual cycle. Addition of both extracts separately inhibited the LH-stimulated in vitro progesterone synthesis by granulosa cell cultures and by incubated sheep corpus luteum slices. These findings provide evidence for the presence of LHRBI in human corpus luteum.
检测冷冻人黄体的水提取物中是否存在促黄体生成素受体位点结合抑制剂（LHRBI），以及其对促黄体生成素（LH）刺激绵羊卵巢细胞合成孕酮的反应的后续影响。在正常月经周期的人黄体提取物（30,000 g 上清液）存在的情况下，125I 人绒毛膜促性腺激素（hCG）与绵羊卵巢颗粒细胞和黄体细胞的结合显著减少，但大鼠睾丸 LH 受体结合标记 hCG 的能力受影响较小。然而，与正常月经周期的黄体提取物相比，妊娠头三个月黄体的提取物对 LH/hCG 与卵巢细胞结合的抑制作用似乎较小，对大鼠睾丸细胞的结合没有影响。分别添加两种提取物均可抑制颗粒细胞培养物和孵育的绵羊黄体切片中 LH 刺激的体外孕酮合成。这些发现为人类黄体中存在 LHRBI 提供了证据。
