McGrath B M, Stevely W S
J Gen Virol. 1980 Aug;49(2):323-32. doi: 10.1099/0022-1317-49-2-323.
The translation in vitro of mRNA from pseudorabies virus infected cells was studied using systems derived from wheat germ and from rabbit reticulocyte. The mRNA was shown by molecular hybridization to contain sequences complementary to virus DNA. Products of in vitro translation co-migrating with virus proteins on polyacrylamide gel electropherograms were detected and the major immune precipitation. Optimum conditions for the stimulation of amino acid incorporation in vitro were determined and found to be similar for mRNA from both infected and mock-infected cells.
利用源自小麦胚芽和兔网织红细胞的系统，研究了来自伪狂犬病病毒感染细胞的mRNA的体外翻译。通过分子杂交表明，该mRNA含有与病毒DNA互补的序列。在聚丙烯酰胺凝胶电泳图上检测到与病毒蛋白共迁移的体外翻译产物，并进行了主要的免疫沉淀。确定了体外刺激氨基酸掺入的最佳条件，发现感染细胞和模拟感染细胞的mRNA的最佳条件相似。
