通过无细胞提取物将来自猴病毒40感染细胞的mRNA翻译为猴病毒40衣壳蛋白。
Translation of mRNA from simian virus 40-infected cells into simian virus 40 capsid protein by cell-free extracts.
作者信息
Lodish H F, Weinberg R, Ozer H L
出版信息
J Virol. 1974 Mar;13(3):590-5. doi: 10.1128/JVI.13.3.590-595.1974.
Abstract
Messenger RNA was isolated from simian virus 40 (SV40)-infected and mock-infected cells by chromatography on poly(U) sepharose. When added to cell-free extracts from Chinese hamster ovary cells or rabbit reticulocytes, RNA from the infected cells, but not from mock-infected cells, stimulated synthesis of the major SV40 capsid protein. Identification of this species was done by sodium dodecyl sulfate gel electrophoresis, peptide mapping, and immunoprecipitation. The in vitro synthesized capsid protein was slightly different from virion assembled capsid protein, as shown by separation upon chromatography on hydroxylapatite and by minor differences in the peptide map.
摘要
通过在聚（U）琼脂糖上进行层析，从感染猿猴病毒40（SV40）的细胞和模拟感染的细胞中分离出信使核糖核酸。当将来自感染细胞而非模拟感染细胞的核糖核酸添加到中国仓鼠卵巢细胞或兔网织红细胞的无细胞提取物中时，会刺激主要SV40衣壳蛋白的合成。通过十二烷基硫酸钠凝胶电泳、肽图谱分析和免疫沉淀来鉴定该蛋白。如通过羟基磷灰石层析分离以及肽图谱中的微小差异所示，体外合成的衣壳蛋白与病毒体组装的衣壳蛋白略有不同。
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