Prives C L, Aviv H, Paterson B M, Roberts B E, Rozenblatt S, Revel M, Winocour E
Proc Natl Acad Sci U S A. 1974 Feb;71(2):302-6. doi: 10.1073/pnas.71.2.302.
Extracts of wheat germ are capable of synthesizing the major capsid protein of simian virus 40. Poly(A)-containing RNA from BS-C-1 cells infected with simian virus 40 directed the synthesis of a novel polypeptide that migrates in polyacrylamide gels together with the major capsid polypeptide of simian virus 40, VP-1. The patterns of the major tryptic peptides of purified VP-1 and the novel polypeptide synthesized in vitro were identical after two-dimensional paper electrophoresis. The novel polypeptide was not synthesized in response to poly(A)-rich RNA from uninfected cells or from virus-infected cells treated with cytosine arabinoside. Messenger RNA from infected cells purified by selective hybridization to DNA of simian virus 40 directs the synthesis of a major polypeptide of electrophoretic mobility similar to that of VP-1 of simian virus 40. This approach should prove useful in identifying additional products specified by DNA tumor viruses.
小麦胚芽提取物能够合成猴病毒40的主要衣壳蛋白。来自感染猴病毒40的BS - C - 1细胞的含聚腺苷酸（Poly(A)）的RNA指导合成一种新型多肽，该多肽在聚丙烯酰胺凝胶中与猴病毒40的主要衣壳多肽VP - 1一起迁移。经过二维纸电泳后，纯化的VP - 1和体外合成的新型多肽的主要胰蛋白酶肽图谱相同。未感染细胞或用阿糖胞苷处理的病毒感染细胞的富含Poly(A)的RNA不会指导合成这种新型多肽。通过与猴病毒40的DNA进行选择性杂交纯化的感染细胞的信使RNA指导合成一种电泳迁移率与猴病毒40的VP - 1相似的主要多肽。这种方法在鉴定DNA肿瘤病毒指定的其他产物方面应该会证明是有用的。
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